Genotyping and primer details


Genotyping and primer details for SALK_067271c - At1g61790
Line ID SALK_067271c
Locus At1g61790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22814427 of line SALK_067271c Chr1:22814427
   
Insertion allele amplicon LBb3 - 36lm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36lm: 5'-CCTGATAATGTTGTGCATTGTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22815130
   
wt amplicon (over InsPos) 05ph - 36lm
wt amplicon start Chr1:22814166
wt amplicon end Chr1:22815130
wt amplicon size 965 bp
Locus specific primer 36lm: 5'-CCTGATAATGTTGTGCATTGTACC-3'
2nd locus specific primer 05ph: 5'-ATGTCTCGTCAAAACTCATAGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11