Genotyping and primer details


Genotyping and primer details for SALK_067473c - At1g06040
Line ID SALK_067473c
Locus At1g06040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1829357 of line SALK_067473c Chr1:1829357
   
Insertion allele amplicon LBb3 - 86by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 86by: 5'-CATCAAAGCATTAAGCATAGACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1828461
   
wt amplicon (over InsPos) 04dd - 86by
wt amplicon start Chr1:1828461
wt amplicon end Chr1:1829431
wt amplicon size 971 bp
Locus specific primer 86by: 5'-CATCAAAGCATTAAGCATAGACCA-3'
2nd locus specific primer 04dd: 5'-GGATTCGTTAGTTTGAATCTGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11