Genotyping and primer details


Genotyping and primer details for SALK_067872c - At1g72740
Line ID SALK_067872c
Locus At1g72740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27382863 of line SALK_067872c Chr1:27382863
   
Insertion allele amplicon LBb3 - 84cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 84cs: 5'-CAGGGATCAACTCAGAAGGTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27381959
   
wt amplicon (over InsPos) 11dz - 84cs
wt amplicon start Chr1:27381959
wt amplicon end Chr1:27382928
wt amplicon size 970 bp
Locus specific primer 84cs: 5'-CAGGGATCAACTCAGAAGGTAAAG-3'
2nd locus specific primer 11dz: 5'-ATTCGATTTAATTTCGAGAGCGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11