Sequences from confirmation amplicon


Confirmation sequences for line SALK_067872c and hit At1g72740 are shown.
Line ID SALK_067872c
Locus At1g72740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27382863
Primers used for PCR 84cs - LBb3, show primer details
   
Sequence Name 88-K109033-SALK_067872C-84cs
GenBank Accession LN486315 [GenBank]
Sequencing primer gene specific primer, 84cs: 5'-CAGGGATCAACTCAGAAGGTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K109033-SALK_067872C-84cs
GAATAGGCCGTGGAATAGTGACAGCATCATTTGCATCTGCAGCCGGAGCCGTCTACTTCC
TCTTTGACTTTTGCTGGCCGTGCCTTATTAGTTAAACTTTGAGTACCAGGGGGAACACTC
AAGTTACGCCACTTATCCTATTAAGTATTGAAACACACAAAATTTTCAAACTCTTCTTCA
TAAGACTACCACCAAGTAAAAGAAATCCATAGCAAAATGAAGGACAGTACTACTCTTTCC
ACCTCAAATCTCCAACTCAACCAAGCTAGCTTTAGGTATTGCACACTTGAATCAATAAAA
AAAAAAACTTATAATCTATCCAAGGAATCTCTAATCCTATAACCAATGATAAGAGGCAAC
CCTAATAAAACCTATTGCCAAAAGTTTTGATTTTTTATGGATCTTAAACTAAGCAGATAC
GACAGAGTACATAATGCACTCCGGGTTGGTCCCAAATTCAATGTCTAGGTTTAATAAATG
GTTCACCGAGAGTGATTTTAAAGAGGTGAGTGAAGAAAAGAGAAAC
   
Sequence Name 72-K109032-SALK_067872C-R210
GenBank Accession LN486050 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K109032-SALK_067872C-R210
GTCATTTGTAATATCGAAAAGTTTTGCAAACTGGAAACCTCCTCCCGCAGTCACCTTCTG
CGCAGAGCCAAAAATAAACGAAACCTCCGGCGAAAGCTACGGCGAAGAGACTGCGTCTAA
CTCCGTTTACACGCCGATTGTCTTGGAGAGAGAGAGAGAGCACGAGGTAAATCACGCAAG
GGTTAGATGGGAAACCAGAAACTAAAGTGGACGGCGGAGGAAGAGGAGGCGTTACTCGCC
GGAATCAGAAAGCACGGTCCTGGAAAGTGGAAGAATATTCTCCGGGATCCAGAATTCGCT
GATCAGCTCATCCATCGCTCCAACATTGACCTAAAGGTTTCTCTTTTCTTCACTCACCTC
TTTAAAATCACTCTCGTTGAACCATATATTAAACCTAGACATCGAATTTGAGACCAACCC
GGAGTGCATTATGTACTCTGTCGTATCTGCTTAGTTTAAGATCCATAAAAAATCAAAACT
TTTGGCAATAGGTTTTATTAGTGTTGCCTCTTATCATTGGTTATAGGATTAGAGATTCCT
TGGATAGATTATAAGTTTTTTTTTTTATTGATTCAAGGGGGCAATACCTAAAGCTAGCTT
GGTTGAGTTGGAGATTTGAGGTGGAA

Last Updated on 06.12.2018 16:11