Genotyping and primer details


Genotyping and primer details for SALK_067994c - At1g16460
Line ID SALK_067994c
Locus At1g16460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5620083 of line SALK_067994c Chr1:5620083
   
Insertion allele amplicon LBb3 - 35gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35gz: 5'-ATCAATCTCCACAAAGATGAGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5619674
   
wt amplicon (over InsPos) 04ix - 35gz
wt amplicon start Chr1:5619674
wt amplicon end Chr1:5620449
wt amplicon size 776 bp
Locus specific primer 35gz: 5'-ATCAATCTCCACAAAGATGAGACC-3'
2nd locus specific primer 04ix: 5'-GGTTTTAAGTTGATTCCGCTCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11