Genotyping and primer details


Genotyping and primer details for SALK_068206 - At1g72830
Line ID SALK_068206
Locus At1g72830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27406133 of line SALK_068206 Chr1:27406133
   
Insertion allele amplicon LBb3 - 35kq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35kq: 5'-CTACCACAGGCACCAGTAAGTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27406446
   
wt amplicon (over InsPos) 47vw - 35kq
wt amplicon start Chr1:27405558
wt amplicon end Chr1:?
wt amplicon size 889 bp
Locus specific primer 35kq: unknown
2nd locus specific primer 47vw: 5'-TTGGTCGTTCTTGTGATGTCTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11