Sequences from confirmation amplicon


Confirmation sequences for line SALK_068206 and hit At1g72830 are shown.
Line ID SALK_068206
Locus At1g72830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27406133
Primers used for PCR 35kq - LBb3, show primer details
   
Sequence Name 49-K117945-SALK_068206C-35kq
GenBank Accession LN485487 [GenBank]
Sequencing primer gene specific primer, 35kq: 5'-CTACCACAGGCACCAGTAAGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K117945-SALK_068206C-35kq
CCTTTCGTTTATCTGTCCATCAGATCTCAAGTCTCTTAATCTCTTTTGCAGACATGCAAT
CCTCAAATGGTGAGTATGATTCCTGGTCGTGTTCCTTTACCAGCAGAGCTCACAGAAACT
GATCCAGTCTTTGTCAATGCGAAGCAATACCACGCAATTATGAGGAGGAGACAGCAACGT
GCTAAGCTTGAGGCTCAAAACAAACTAATCAGAGCCCGTAAGGTAGTTACCAAATTTTGG
TTTATATTTTAAACAAACGCATTGACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTACGAAATCGGCAAAAT
   
Sequence Name 14-K117944-SALK_068206C-R210
GenBank Accession LN484681 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117944-SALK_068206C-R210
GTCATTTGTCATGCGTTTGTTTAAATATAAACCAAAATTTGGTAACTACCTTACGGGCTC
TGATTAGTTTGTTTTGAGCCTCAAGCTTAGCACGTTGCTGTCTCCTCCTCATAATTGCGT
GGTATTGCTTCGCATTGACAAAGACTGGATCAGTTTCTGTGAGCTCTGCTGGTAAAGGAA
CACGACCAGGAATCATACTCACCATTTGAGGATTGCATGTCTGCAAAAGAGATTAAGAGA
CTTGAGATCTGATGGACAGATAAAACTGAAAGGTAAATCTCCCACTTGGAAACGAAGGGT
GTACTTACTGGTGCCTGTGGTAG

Last Updated on 06.12.2018 16:11