Genotyping and primer details


Genotyping and primer details for SALK_068236 - At1g53770
Line ID SALK_068236
Locus At1g53770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20072119 of line SALK_068236 Chr1:20072119
   
Insertion allele amplicon LBb3 - 23hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23hd: 5'-GGGGATGTGTTCTATGCTAATGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20072634
   
wt amplicon (over InsPos) 10il - 23hd
wt amplicon start Chr1:20071741
wt amplicon end Chr1:20072634
wt amplicon size 894 bp
Locus specific primer 23hd: 5'-GGGGATGTGTTCTATGCTAATGTT-3'
2nd locus specific primer 10il: 5'-TGTCTGTATAACAATGCATCCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11