Genotyping and primer details


Genotyping and primer details for SALK_068675c - At5g20480
Line ID SALK_068675c
Locus At5g20480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6924956 of line SALK_068675c Chr5:6924956
   
Insertion allele amplicon R204 - BL07
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL07: 5'-GAATGTTGACTTCTCCAACAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6924185
   
wt amplicon (over InsPos) BL08 - BL07
wt amplicon start Chr5:6924185
wt amplicon end Chr5:6925266
wt amplicon size 1082 bp
Locus specific primer BL07: 5'-GAATGTTGACTTCTCCAACAACAA-3'
2nd locus specific primer BL08: 5'-AACAACACACAATGAGGTTGGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11