Sequences from confirmation amplicon


Confirmation sequences for line SALK_068675c and hit At5g20480 are shown.
Line ID SALK_068675c
Locus At5g20480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6924956
Primers used for PCR BL07 - R204, show primer details
   
Sequence Name 68-K105636-SALK_068675C-BL07
GenBank Accession LN485943 [GenBank]
Sequencing primer gene specific primer, BL07: 5'-GAATGTTGACTTCTCCAACAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K105636-SALK_068675C-BL07
TCTTCCTTCGCTGCGAATCTGAATCTTTCTATGAACAAGTTTGAGGGAAGGGTGCCAACA
ACAGGAGTGTTTCGAAATGCTACAGCAGTTTCTGTTTTTGGTAACACAAATATTTGCGGA
GGCGTCCGAGAAATGCAACTAAAGCCATGCATTGTACAGGCATCTCCAAGGAAGAGAAAG
CCTCTGTCAGTTAGAAAGAAAGTTGTCAGTGGTATTTGTATAGGTATAGCTTCGCTTTTG
TTAATCATAATTGTGGCTTCTCTGTGTTGGTTCATGAAGAGGAAAAAGAAAAACAATGCC
AGTGATGGTAACCCATCTGATTCTACTACTTTGGGGATGTTCCATGAGAAGGTAAGTTAT
GAAGAGCTTCATAGTGCAACAAGTCGCTTCTCTTCAACCAATTTGATTGGTTCAGGCAAT
TTCGGTAATGTGTTTAAAGGATTGCTTGGCCCTGAGAATAAACTCGTCGCGGTTAAAGTT
TTGAACCTCCTAAAGCATGGAGCGACGAAAAGCTTTATGGCGGAATGTGAAACCTTCAAG
GGTATACGACATCGTAACCTTGTAAAACTGATAACGGTTTGTTCAAGCCTTGATTCCGAG
GGAAATGATTTCAGAGCTCTGGTCTATGAGTTCATGCCAAAAGGAAGTCTGGATATGTGG
CTGCAGCTAGAAGATCTGGAAAGGGTAAACGATCACTCGAGATCTTTAACACCCCAGGAT
ATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCACA
   
Sequence Name 72-K105637-SALK_068675C-R210
GenBank Accession LN486046 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K105637-SALK_068675C-R210
GTCATTTGTTACCCACAATATATCCTGGGGTGTTAAAGATCTCGAGTGATCGTTTACCCT
TTCCAGATCTTCTAGCTGCAGCCACATATCCAGACTTCCTTTTGGCATGAACTCATAGAC
CAGAGCTCTGAAATCATTTCCCTCGGAATCAAGGCTTGAACAAACCGTTATCAGTTTTAC
AAGGTTACGATGTCGTATACCCTTGAAGGTTTCACATTCCGCCATAAAGCTTTTCGTCGC
TCCATGCTTTAGGAGGTTCAAAACTTTAACCGCGACGAGTTTATTCTCAGGGCCAAGCAA
TCCTTTAAACACATTACCGAAATTGCCTGAACCAATCAAATTGGTTGAAGAGAAGCGACT
TGTTGCACTATGAAGCTCTTCATAACTTACCTTCTCATGGAACATCCCCAAAGTAGTAGA
ATCAGATGGGTTACCATCACTGGCATTGTTTTTCTTTTTCCTCTTCATGAACCAACACAG
AGAAGCCACAATTATGATTAACAAAAGCGAAGCTATACCTATACAAATACCACTGACAAC
TTTCTTTCTAACTGACAGAGGCTTTCTCTTCCTTGGAGATGCCTGTACAATGCATGGCTT
TAGTTGCATTTCTCGGACGCCTCCGCAAATATTTGTGTTACCAAAAACAGAAACTGCTGT
AGCATTTCGAAACACTCCTGTTGTTGGCACCCTTCCCTCAAACTTGTTCATAGAAAGATT
CAGATTTCGCAGCGAAGGAAGACTGGCCAGATATCGAGGTATCCGGCCAGAGAGATTGTT
GTTGGA

Last Updated on 06.12.2018 16:11