Genotyping and primer details


Genotyping and primer details for SALK_068690c - At3g53960
Line ID SALK_068690c
Locus At3g53960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19978221 of line SALK_068690c Chr3:19978221
   
Insertion allele amplicon LBb3 - 21ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21ht: 5'-AAGGAACACCACATGAATAAGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19978761
   
wt amplicon (over InsPos) 15ix - 21ht
wt amplicon start Chr3:19977859
wt amplicon end Chr3:19978761
wt amplicon size 903 bp
Locus specific primer 21ht: 5'-AAGGAACACCACATGAATAAGACC-3'
2nd locus specific primer 15ix: 5'-CTTGTTGCCTTATTCTTGTCCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11