Sequences from confirmation amplicon


Confirmation sequences for line SALK_068690c and hit At3g53960 are shown.
Line ID SALK_068690c
Locus At3g53960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19978221
Primers used for PCR 21ht - LBb3, show primer details
   
Sequence Name 79-K112669-SALK_068690C-21ht
GenBank Accession LN486207 [GenBank]
Sequencing primer gene specific primer, 21ht: 5'-AAGGAACACCACATGAATAAGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K112669-SALK_068690C-21ht
CTCTCATTCCTAGTCTTAGGAGTTGCGGATGCTTTTACCCTTGTCGGTCTTCAAGAATAT
TTCTACGACCAAGTCCCAGATTCTATGAGAAGCTTAGGCATAGCGTTTTACCTCAGCGTG
CTTGGAGCGGCTAGCTTTGTCAACAATCTTTTGATAACGGTTAGTGATCATTTAGCCGAG
GAAATTTCCGGGAAGGGCTGGTTTGGGAAAGACCTTAATAGCAGCCGCTTGGACCGCTTT
TACTGGATGCTAGCCGCCTTGACCGCTGCAAATATATGCTGCTTTGTGATCGTGGCCATG
AGATACACTTACAAGACTGTGCAGCCGAGTCTGGCTGTTGTTGCTGACGGCGGTGATGAC
GTTGAGACAGCCACGGGGACGAATAACACGTCCAAGTTTACGTAACTTAGCTATTGGTTT
GGTCATATGATGACCATTTTATGTTCTGTGTTTATTGTGTACTTTTGAAAAGTGATTTTG
TAGTCATTTTGTATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 69-K112668-SALK_068690C-R210
GenBank Accession LN485978 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K112668-SALK_068690C-R210
CGTCATTTGTTACACCACAATATATACAAAATGACTACAAAATCACTTTTCAAAAGTACA
CAATAAACACAGAACATAAAATGGTCATCATATGACCAAACCAATAGCTAAGTTACGTAA
ACTTGGACGTGTTATTCGTCCCCGTGGCTGTCTCAACGTCATCACCGCCGTCAGCAACAA
CAGCCAGACTCGGCTGCACAGTCTTGTAAGTGTATCTCATGGCCACGATCACAAAGCAGC
ATATATTTGCAGCGGTCAAGGCGGCTAGCATCCAGTAAAAGCGGTCCAAGCGGCTGCTAT
TAAGGTCTTTCCCAAACCAGCCCTTCCCGGAAATTTCCTCGGCTAAATGATCACTAACCG
TTATCAAAAGATTGTTGACAAAGCTAGCCGCTCCAAGCACGCTGAGGTAAAACGCTATGC
CTAAGCTTCTCATAGAATCTGGGACTTGGTCGTAGAAATATTCTTGAAGACCGACAAGGG
TAAAAGCATCCGCAACTCCTAAGACTAGGAATTGAGGAGCTAACCATATAGCACTCAAGG
TCATGGTCTTATTCATGTGGTGTTCCT

Last Updated on 06.12.2018 16:11