Genotyping and primer details


Genotyping and primer details for SALK_069091c - At5g57360
Line ID SALK_069091c
Locus At5g57360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23243415 of line SALK_069091c Chr5:23243415
   
Insertion allele amplicon LBb3 - 22ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22ht: 5'-ATCTTTCTTCCTCCATAAACCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23243814
   
wt amplicon (over InsPos) 02jm - 22ht
wt amplicon start Chr5:23242893
wt amplicon end Chr5:23243814
wt amplicon size 922 bp
Locus specific primer 22ht: 5'-ATCTTTCTTCCTCCATAAACCGAT-3'
2nd locus specific primer 02jm: 5'-TTATCGAGACAGACATTGACCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11