Genotyping and primer details


Genotyping and primer details for SALK_069718 - At4g14950
Line ID SALK_069718
Locus At4g14950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8547279 of line SALK_069718 Chr4:8547279
   
Insertion allele amplicon LBb3 - CE27
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE27: 5'-GCTTCCTCCATATTTCATCTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8546592
   
Insertion allele amplicon R212 - CE28
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CE28: 5'-CGCAAATTCAGTTGATCTTTTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8547546
   
wt amplicon (over InsPos) CE28 - CE27
wt amplicon start Chr4:8546592
wt amplicon end Chr4:8547546
wt amplicon size 955 bp
Locus specific primer CE27: 5'-GCTTCCTCCATATTTCATCTCAAG-3'
2nd locus specific primer CE28: 5'-CGCAAATTCAGTTGATCTTTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11