Genotyping and primer details


Genotyping and primer details for SALK_070821c - At1g56080
Line ID SALK_070821c
Locus At1g56080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20976196 of line SALK_070821c Chr1:20976196
   
Insertion allele amplicon LBb3 - 33de
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33de: 5'-TTCTGACATCAGCAGTCTCAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20975518
   
wt amplicon (over InsPos) 12dz - 33de
wt amplicon start Chr1:20975518
wt amplicon end Chr1:20976458
wt amplicon size 941 bp
Locus specific primer 33de: 5'-TTCTGACATCAGCAGTCTCAGTTT-3'
2nd locus specific primer 12dz: 5'-TTCAAGTTCTTGGGGTTTAAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11