Genotyping and primer details


Genotyping and primer details for SALK_071698c - At1g55320
Line ID SALK_071698c
Locus At1g55320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20635359 of line SALK_071698c Chr1:20635359
   
Insertion allele amplicon LBb3 - 50wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50wm: 5'-CTTCCTATGATGTATGACGAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20635665
   
wt amplicon (over InsPos) 36xa - 50wm
wt amplicon start Chr1:20634780
wt amplicon end Chr1:20635665
wt amplicon size 886 bp
Locus specific primer 50wm: 5'-CTTCCTATGATGTATGACGAAGCA-3'
2nd locus specific primer 36xa: 5'-ACAGGATGTATCATGGATGGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11