Sequences from confirmation amplicon


Confirmation sequences for line SALK_071698c and hit At1g55320 are shown.
Line ID SALK_071698c
Locus At1g55320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20635359
Primers used for PCR 50wm - LBb3, show primer details
   
Sequence Name 47-K118709-SALK_071698C-50wm
GenBank Accession LN485447 [GenBank]
Sequencing primer gene specific primer, 50wm: 5'-CTTCCTATGATGTATGACGAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118709-SALK_071698C-50wm
TTTGTATCAGCTTTAGAAGAAGCCAGAGAACATCGTCAACATTGGAAGCTTCTCCTGTAG
TGGCGAAAAACCTAACAAAACACAACAGCTTCTAAGTTAAAATTTCTCGTATAAACCCGA
AAACAAAACAAGGTTTGTTGCTCCTTACTTAATCTTTGTCCAATTAAGCCCTTCCATACA
GTTTGTTCTCTTCCAAGTTTTCACCAAGCTTGGAACAGTACCAAGAACTGTAACTCCAGC
ATCCTGAAGTAATATCAAGCACAAATAAGTGAGGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCAGAATCGNGCAAAAT
   
Sequence Name 22-K118708-SALK_071698C-R210
GenBank Accession LN484931 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118708-SALK_071698C-R210
CTTCAGGATGCTGGAGTTCANTTCTTGGTACTGTTCCAAGCTTGGTGAAAACTTGGAAGA
GAACAAACTGTATGGAAGGGCTTAATTGGACAAAGATTAAGTAAGGAGCAACAAACCTTG
TTTTGTTTTCGGGTTTATACGAGAAATTTTAACTTAGAAGCTGTTGTGTTTTGTTAGGTT
TTTCGCCACTACAGGAGAAGCTTCCAATGTTGACGATGTTCTCTGGCTTTCTTCTAAAGC
TGATTACAAACCTGTGATCGAATGCTGTGGAGGCACGGAACTTGCTTCGTCATACATCAT
AGGAAG

Last Updated on 06.12.2018 16:11