Genotyping and primer details


Genotyping and primer details for SALK_072274c - At5g63510
Line ID SALK_072274c
Locus At5g63510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25425035 of line SALK_072274c Chr5:25425035
   
Insertion allele amplicon LBb3 - 19gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19gg: 5'-CCTTGAAGAAATCCAAAATCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25424027
   
wt amplicon (over InsPos) 10ho - 19gg
wt amplicon start Chr5:25424027
wt amplicon end Chr5:25425119
wt amplicon size 1093 bp
Locus specific primer 19gg: 5'-CCTTGAAGAAATCCAAAATCCATA-3'
2nd locus specific primer 10ho: 5'-GATACTGTTCCGGGTCAAATAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11