Genotyping and primer details


Genotyping and primer details for SALK_072332c - At5g05850
Line ID SALK_072332c
Locus At5g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1763424 of line SALK_072332c Chr5:1763424
   
Insertion allele amplicon LBb3 - 89ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 89ns: 5'-CTTCATATTCGCCATCTTCTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1762823
   
wt amplicon (over InsPos) 20rd - 89ns
wt amplicon start Chr5:1762823
wt amplicon end Chr5:1763699
wt amplicon size 877 bp
Locus specific primer 89ns: 5'-CTTCATATTCGCCATCTTCTCTTC-3'
2nd locus specific primer 20rd: 5'-TTTGCTCTGGATTTTGAGTCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11