Genotyping and primer details


Genotyping and primer details for SALK_072886 - At1g54200
Line ID SALK_072886
Locus At1g54200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20235874 of line SALK_072886 Chr1:20235874
   
Insertion allele amplicon LBb3 - 33dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33dh: 5'-TGTTCTTGTTGTTGTACTTGGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20236638
   
wt amplicon (over InsPos) 14dz - 33dh
wt amplicon start Chr1:20235621
wt amplicon end Chr1:20236638
wt amplicon size 1018 bp
Locus specific primer 33dh: 5'-TGTTCTTGTTGTTGTACTTGGAGG-3'
2nd locus specific primer 14dz: 5'-GGCAATAACACTATTTTGGCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11