Sequences from confirmation amplicon


Confirmation sequences for line SALK_072886 and hit At1g54200 are shown.
Line ID SALK_072886
Locus At1g54200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20235874
Primers used for PCR 33dh - LBb3, show primer details
   
Sequence Name 27-K109819-SALK_072886C-33dh
GenBank Accession LN485057 [GenBank]
Sequencing primer gene specific primer, 33dh: 5'-TGTTCTTGTTGTTGTACTTGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K109819-SALK_072886C-33dh
GGAAAACGTCGGATCTTTTCGATTTTTCGCTGACGATGGCGTTTTGCTCAGCAAGATCTG
GAGAATGATGAAGCAGAGGAGCATGTAGTGGTTGATGACGCTGCGGCGGCGGCGGCGGTG
GTGGAAGTGACGGTTGTGTTGATTTTGTTAAGTTTTTTAGTGTTTCCGGCGCCGGTGAAT
ATGGAGTTAAGGAAAGTAGCGAGACGACCACCGGGAGAAATCGGTTGTTTCACTTTCTTC
AAATCGCTATAAATCTTCAACGCTTTAGATTTCGTTTTGAGAAAGCTTCCATGTTCTTGT
TTATTACTACTGTTAGGTCGGTGATTCTGAGGAGATCTTTCGAATCGTTCCACGGTGGTT
CGTATCGGTTTAGGATGAGAAATCGCCGGCGAGGATTTAGATCGTCTGTAGAAATAATCA
GACTCGGAAGACGAGAATCCGCTTGAGTCAGAGGAGCTTGAGCTTGAATGTTTGAAGAAA
ACAGGTTCTATGGTTTTAAGATTCTTGGATCTGTTGAAATCGTCGCGGCGGTTAGCAGAA
ACACGCGTGTCCTCGCGGTTCTGATGTTTGGTTT
   
Sequence Name 11-K109818-SALK_072886C-R210
GenBank Accession LN484579 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109818-SALK_072886C-R210
CGTCATTTGTTTACCCACAATAACATACCACTAGACCACCAACACCGTCTCCGTAACCGC
CGTGACCACCGTCATCCTTCCTTCTCCTCTACTCTCCTCGACCAAATTTACCGCTCCATC
GACGATTCCTCTACTAACTCCTCATCCATGAGAAAAACCAAACATCAGAACCGCGAGGAC
ACGCGTGTTTCTGCTAACCGCCGCGACGATTTCAACAGATCCAAGAATCTTAAAACCATA
GAACCTGTTTTCTTCAAACATTCAAGCTCAAGCTCCTCTGACTCAAGCGGATTCTCGTCT
TCCGAGTCTGATTATTTCTACAGACGATCTAAATCCTCGCCGGCGATTTCTCATCCTAAA
CCGATACGAACCACCGTGGAACGATTCGAAAGATCTCCTCAGAATCACCGACCTAACAGT
AGTAATAAACAAGAACATGGAAGCTTTCTCAAAACGAAATCTAAAGCGTTGAAGATTTAT
AGCGATTTGAAGAAAGTGAAACAACCGATTTCTCCCGGTGGTCGTCTCGCTACTTTCCTT
AACTCCATATTCACCGGCGCCGGAAACACTAAAAAACTTAACAAAATCAACACAACCGTC
ACTTCCACCACCGCCGCCGCCGCCGCAGCGTCATCAACCACTACATGCTCCTCTGCTTCA
TCATTCTCCAGATCTTGCTTGAGCAAAACGCCATCGTCTAGCGAAAAATCGAAAAGATCC
GTACGTTTTTGTCCCGTCAACGTCATCTTCG

Last Updated on 06.12.2018 16:11