Genotyping and primer details

Genotyping and primer details for SALK_072930c - At2g22540
Line ID SALK_072930c
Locus At2g22540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9582644 of line SALK_072930c Chr2:9582644
Insertion allele amplicon LBb3 - 42fx
Locus specific primer 42fx: 5'-TATAGGCAAATGAGAGGAGAGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9582046
wt amplicon (over InsPos) 10hf - 42fx
wt amplicon start Chr2:9582046
wt amplicon end Chr2:9582849
wt amplicon size 804 bp
Locus specific primer 42fx: 5'-TATAGGCAAATGAGAGGAGAGGAA-3'
2nd locus specific primer 10hf: 5'-ATTTTGTGTTTTTAGTGGCACCAT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11