Sequences from confirmation amplicon


Confirmation sequences for line SALK_072930c and hit At2g22540 are shown.
Line ID SALK_072930c
Locus At2g22540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9582644
Primers used for PCR 42fx - LBb3, show primer details
   
Sequence Name 64-K111812-SALK_072930C-42fx
GenBank Accession LN485847 [GenBank]
Sequencing primer gene specific primer, 42fx: 5'-TATAGGCAAATGAGAGGAGAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K111812-SALK_072930C-42fx
AGAGGCCCTTGAACTGGTTTGACGCGTGTGATTGAACAAAGGTTGTTAAGAAAATTACTT
GATACCATGTATAAGTTTCTCTAAGCTTACGAGTATGCAATTTACTAATACGAGATGTGT
TTGCAGAGTGACAAGATTATGAGTGAGATCAGCGAACTTCAGAAAAAGGTAATAATTAAC
CAAAATAACGTTTATTCTTTACTTGATGATTTCAATATTAATTTTGGCAGTTTCAAGATC
CAAAATTTTCATCTTCTTCTCTTTTTTTTTGGTGTTCAGGGAATGCAATTGATGGATGAG
AACAAGCGGTTGAGGCAGCAAGTATGTGTCTTACCCTCTCTGTTGATAACAAATCCCTTT
CTTTTGTCTACCATTAACGTACACACCCCTAAATTTAATCCCCAGTTGTCTACAACACAT
ATGTTTGATCATACTGTGAGATAAATGAATAAACCAAGTGATATAGCGCGATTTAAAAAT
GTCTTTAAAACTAAAGGTAACCATGTAGCTNGTTAGTCTCTAGGGTCCTAGAGGTGCGGA
TGGCTGAAGGCGGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCC
   
Sequence Name 54-K111811-SALK_072930C-R210
GenBank Accession LN485604 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K111811-SALK_072930C-R210
GTCGCCTTCGCCACCGCACCTCTAGGACCCTAGAGACTAACTAGCTACATGGTTACCTTT
AGTTTTAAAGACATTTTTAAATCGCGCTATATCACTTGGTTTATTCATTTATCTCACAGT
ATGATCAAACATATGTGTTGTAGACAACTGGGGATTAAATTTAGGGGTGTGTACGTTAAT
GGTAGACAAAAGAAAGGGATTTGTTATCAACAGAGAGGGTAAGACACATACTTGCTGCCT
CAACCGCTTGTTCTCATCCATCAATTGCATTCCCTGAACACCAAAAAAAAAGAGAAGAAG
ATGAAAATTTTGGATCTTGAAACTGCCAAAATTAATATTGAAATCATCAAGTAAAGAATA
AACGTTATTTTGGTTAATTATTACCTTTTTCTGAAGTTCGCTGATCTCACTCATAATCTT
GTCACTCTGCAAACACATCTCGTATTAGTAAATTGCATACTCGTAAGCTTAGAGAAACTT
ATACATGGTATCAAGTAATTTTCTTAACAACCTTTGTTTCAATCACACGCGTCAAACCAG
TTTCAAGGGCCTTCTCTAGCTGCTGAAGCTCTTCAATGTCAAGTCCTTGAAGTTCCTCTC
CT

Last Updated on 06.12.2018 16:11