Genotyping and primer details


Genotyping and primer details for SALK_073252c - At3g04520
Line ID SALK_073252c
Locus At3g04520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1217609 of line SALK_073252c Chr3:1217609
   
Insertion allele amplicon LBb3 - AW25
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AW25: 5'-TTTTATTTTTCAATCCGTGATTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1217127
   
wt amplicon (over InsPos) AW25 - AW26
wt amplicon start Chr3:1217127
wt amplicon end Chr3:1217907
wt amplicon size 781 bp
Locus specific primer AW26: 5'-GTGTTTGAAATGAATGATTGGTTG-3'
2nd locus specific primer AW25: 5'-TTTTATTTTTCAATCCGTGATTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11