Genotyping and primer details


Genotyping and primer details for SALK_073422 - At1g30810
Line ID SALK_073422
Locus At1g30810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10938356 of line SALK_073422 Chr1:10938356
   
Insertion allele amplicon LBb3 - 59cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 59cs: 5'-TTCTGCATCCACAGTACATTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10937645
   
wt amplicon (over InsPos) 26dw - 59cs
wt amplicon start Chr1:10937645
wt amplicon end Chr1:10938535
wt amplicon size 891 bp
Locus specific primer 59cs: 5'-TTCTGCATCCACAGTACATTTCTC-3'
2nd locus specific primer 26dw: 5'-GACGAGAGCTTCTTCAATGTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11