Genotyping and primer details


Genotyping and primer details for SALK_073618 - At2g02970
Line ID SALK_073618
Locus At2g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:867121 of line SALK_073618 Chr2:867121
   
Insertion allele amplicon LBb3 - 26dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26dh: 5'-GAGACTGAAGTTAGGTTAATGGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:867524
   
wt amplicon (over InsPos) 14gc - 26dh
wt amplicon start Chr2:866510
wt amplicon end Chr2:867524
wt amplicon size 1015 bp
Locus specific primer 26dh: 5'-GAGACTGAAGTTAGGTTAATGGCG-3'
2nd locus specific primer 14gc: 5'-ACTGAATGAGTGGAAAGGAAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11