Genotyping and primer details

Genotyping and primer details for SALK_073673 - At5g64080
Line ID SALK_073673
Locus At5g64080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25646571 of line SALK_073673 Chr5:25646571
Insertion allele amplicon LBb3 - 61us
Locus specific primer 61us: 5'-AAGAAAGACAAAATACCTGGAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25645985
wt amplicon (over InsPos) 37vl - 61us
wt amplicon start Chr5:25645985
wt amplicon end Chr5:25646739
wt amplicon size 755 bp
Locus specific primer 61us: 5'-AAGAAAGACAAAATACCTGGAGCA-3'
2nd locus specific primer 37vl: 5'-CTTCACTCTTTCTCATTTTCCACC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11