Sequences from confirmation amplicon


Confirmation sequences for line SALK_073673 and hit At5g64080 are shown.
Line ID SALK_073673
Locus At5g64080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25646571
Primers used for PCR 61us - LBb3, show primer details
   
Sequence Name 10-K117668-SALK_073673C-R210
GenBank Accession LN484567 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117668-SALK_073673C-R210
TTTGTTTCCCACAATATATCCTGGACAAATTGATAGTTTCCCGTCTCAGTTCTCGGCGCA
TCTCACCACCACGCAACGGCGCCGGCTCCGTCTGTAGACTGTTCGACTCTCATACTCAAC
ATGGCTGACTGTTTATCCTTCGTTTCGAGCGGAGGCACGGTGGCGAAACCGGAAGGTACA
TGTTGCTCTGGTCTTAAGACGGTGCTTAAAGCTGACTCTCAGTGTCTATGTGAAGCGTTT
AAGAGCAGTGCTTCTCTTGGAGTTACTTTGAATATCACTAAGGCTTCTACTCTTCCCGCC
GCATGCAAGCTTCACGCTCCTTCTATCGCTACTTGTGGATGTAAGATCTATTCATTTCTT
AACTTGTTTGTTTCTTAGATCTCTTCCGGTTTTGTAGATCTTAATTTGTTATGCTTAATC
TTCGAGAGCGGTTTCTTAAATCCACATTTTTTCTAATTTTTTCAGTTTAATTAAGAAAAT
AATTTGTAGATTTTTCGAAACTGTTTTAAGATTCTATTTTTTGTTGTTGTTGATGTTGAT
TAAATTACTTATAATGCTTTTTTATTTTATTTTTGTTCTTACAGTGTCTGTTGCTCCAAG
TACTGCTCCAGGTAT
   
Sequence Name 47-K117669-SALK_073673C-61us
GenBank Accession LN485442 [GenBank]
Sequencing primer gene specific primer, 61us: 5'-AAGAAAGACAAAATACCTGGAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117669-SALK_073673C-61us
AGACAAAATAAATAAAAAGCATTATAAGTAATTTAATCAACATCAACAACAACACAATTA
GAATCTTAAAACAGTTTCGAAAAATCTACAAATTATTTTCTTAATTAAACTGAAAAAATT
AGAAAAAATGTGGATTTAAGAAACCGCTCTCGAAGATTAAGCATAACAAATTAAGATCTA
CAAAACCGGAAGAGATCTAAGAAACAAACAAGTTAAGAAATGAATAGATCTTACATCCAC
AAGTAGCGATAGAAGGAGCGTGAAGCTTGCATGCGGCGGGAAGAGTAGAAGCCTTAGTGA
TATTCAAAGTAACTCCAAGAGAAGCACTGCTCTTAAACGCTTCACATAGACACTGAGAGT
CAGCTTTAAGCACCGTCTTAAGACCAGAGCAACATGTACCTTCCGGTTTCGCCACCGTGC
CTCCGCTCGAAACGAAGGATAAACAGTCAGCCATGTTGAGTATGAGAGTCGAACAGTCTA
CAGACGGAGCCGGCGCCGTTGCGTGGTGGTGAGATGCGCCGAGAACTGAGACGGGAAACT
ATCAATTTGTCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11