Genotyping and primer details


Genotyping and primer details for SALK_073785 - At2g30790
Line ID SALK_073785
Locus At2g30790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13120028 of line SALK_073785 Chr2:13120028
   
Insertion allele amplicon R204 - 04bc
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 04bc: 5'-AGTATTCTTTCCCTCCGATTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13119229
   
wt amplicon (over InsPos) 37lt - 04bc
wt amplicon start Chr2:13119229
wt amplicon end Chr2:13120409
wt amplicon size 1181 bp
Locus specific primer 04bc: 5'-AGTATTCTTTCCCTCCGATTTCTT-3'
2nd locus specific primer 37lt: 5'-CGGTTTCATGTTGCTCTAATATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11