Genotyping and primer details


Genotyping and primer details for SALK_074400c - At1g78610
Line ID SALK_074400c
Locus At1g78610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29571442 of line SALK_074400c Chr1:29571442
   
Insertion allele amplicon LBb3 - 23ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23ht: 5'-CTTAGGTATGGACGAGCTTCAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29571806
   
wt amplicon (over InsPos) 13ix - 23ht
wt amplicon start Chr1:29570850
wt amplicon end Chr1:29571806
wt amplicon size 957 bp
Locus specific primer 23ht: 5'-CTTAGGTATGGACGAGCTTCAATC-3'
2nd locus specific primer 13ix: 5'-CTGATTCTCTCTTCCTCTTCCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11