Genotyping and primer details


Genotyping and primer details for SALK_075011c - At3g01850
Line ID SALK_075011c
Locus At3g01850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:300548 of line SALK_075011c Chr3:300548
   
Insertion allele amplicon LBb3 - 27di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27di: 5'-CTATGTTGACCCCGAAAATAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:299925
   
wt amplicon (over InsPos) 17ec - 27di
wt amplicon start Chr3:299925
wt amplicon end Chr3:300843
wt amplicon size 919 bp
Locus specific primer 27di: 5'-CTATGTTGACCCCGAAAATAAAAA-3'
2nd locus specific primer 17ec: 5'-TGATGACTGTAGAACCTGGATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11