Genotyping and primer details


Genotyping and primer details for SALK_075267c - At1g55760
Line ID SALK_075267c
Locus At1g55760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20847812 of line SALK_075267c Chr1:20847812
   
Insertion allele amplicon LBb3 - 58qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58qz: 5'-CGCCTCTTTTAAATCAGCAATATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20847348
   
wt amplicon (over InsPos) 15rz - 58qz
wt amplicon start Chr1:20847348
wt amplicon end Chr1:20848376
wt amplicon size 1029 bp
Locus specific primer 58qz: 5'-CGCCTCTTTTAAATCAGCAATATC-3'
2nd locus specific primer 15rz: 5'-ACTCTCCTGTGTTTTTCACCTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11