Sequences from confirmation amplicon


Confirmation sequences for line SALK_075267c and hit At1g55760 are shown.
Line ID SALK_075267c
Locus At1g55760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20847812
Primers used for PCR 58qz - LBb3, show primer details
   
Sequence Name 75-K116055-SALK_075267C-58qz
GenBank Accession LN486135 [GenBank]
Sequencing primer gene specific primer, 58qz: 5'-CGCCTCTTTTAAATCAGCAATATC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K116055-SALK_075267C-58qz
ATCTGTGTATAAAAAGTCTTCGTTTTGGATATTGCCGTAGATATAACTGAGAAAAGCTTG
GCAAGCATCAAGTGGCATGTCGAGTACGTTTATTTCTGATAGTTCTTTCTCTTTCAGGTC
GTGTAAAAACATGCTGCGGAAAACAGGTGAACGGGCAGCGAGAACTGCTCGGTGAGCTCC
AATGCTTCCATCAGAGGCATTGATCGTTATGTCGGTGTAAATGCTTTCTGTCAACATACG
TCCAAGGGATGTTACCGCAGTTACTTGCGATTGATTCTCAGTTGAACCGTTGGCCCAGAT
AGAGTAAAGTTCTCCACTCTGCCGAATTCAGGTAATTTATCAGTTCAGCACACATGTTTC
ACCAAACATACTGATTCAATGTTGCATAGAATAGTTAATTTAAGAAAGAAATATATTGCG
GTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACT
GGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGG
CCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGT
TTGATGGTGGTTCC
   
Sequence Name 48-K116054-SALK_075267C-R210
GenBank Accession LN485459 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116054-SALK_075267C-R210
AATTACCTGAATTCGGCAGAGTGGAGAACTTTACTCTATCGGGGCCGACGGTTCGTCTGA
GAATCAATCGCAAGTAACTGCGGATACCTCCGTTGGACTTATGTGGACAGATAGCATTTA
CACCGACATAGCGATCAATGCCCCTGATGGAAGCGGTGGAGCTCACCGATCCTTTCTCGC
TGCTCTTTCATCTGTTTTCCGCCGCATGTTATTACACGACCTCAACGAGAAAGAACTATC
GGAAATATACGTACTCGACATGCTTCTTGACGCTTGTCCAGCTTTTCTGATTTATATCAA
CGGTAATATCAAAAAGAAATATTTTGTTATGCACAGAT

Last Updated on 06.12.2018 16:11