Genotyping and primer details


Genotyping and primer details for SALK_075450c - At5g56300
Line ID SALK_075450c
Locus At5g56300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22800203 of line SALK_075450c Chr5:22800203
   
Insertion allele amplicon LBb3 - 47vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47vi: 5'-ACCAATCCTTATCTCATCTCTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22799714
   
wt amplicon (over InsPos) 64vw - 47vi
wt amplicon start Chr5:22799714
wt amplicon end Chr5:22800735
wt amplicon size 1022 bp
Locus specific primer 47vi: 5'-ACCAATCCTTATCTCATCTCTCCA-3'
2nd locus specific primer 64vw: 5'-GTATCTTGGTCCCCAAATTGACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11