Sequences from confirmation amplicon


Confirmation sequences for line SALK_075450c and hit At5g56300 are shown.
Line ID SALK_075450c
Locus At5g56300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22800203
Primers used for PCR 47vi - LBb3, show primer details
   
Sequence Name 50-K117945-SALK_075450C-47vi
GenBank Accession LN485517 [GenBank]
Sequencing primer gene specific primer, 47vi: 5'-ACCAATCCTTATCTCATCTCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117945-SALK_075450C-47vi
ACAACTCCCATCAAATCACCGATCTCGGCTGCGCCCCGGCTCCACACGTTTACTACCGTT
GATACGGTGGTTGAGACGTTACAACGGCGATACACTGCCAGGTGCGGTGGTGGTGGGTCG
CCGGAGTTTGAGGCTTTCTTCTGTGATTTGCCTTCTAATGACTTCAACATGTTGTTCAAG
TTGTTGGCTGAGAAACAGAAAGTTGACTCGCCGGCGAAATACTTCGCTGGCGGCGTTGCT
GGTTCGTTCTATGACCGGCTTTTTCCCAGAGGAACGATTCATGTCGCTGTGAGCTTAAGT
GCCTTGCACTGGCTCTCTCAGGTCTAATACACTAATCACTTTTCTATAAATTATTTTTTG
TTACTCTTTATTTATTTATTTATTCATAATATTCACCATTCAAAATAATTAACGACGTCA
ATTTGACATTATAAGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 15-K117944-SALK_075450C-R210
GenBank Accession LN484708 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117944-SALK_075450C-R210
GTCATTTGTTACCCACAATATATCTTATAATGTCAAATTGACGTCGTTAATTATTTTGAA
TGGTGAATATTATGAATAAATAAATAAATAAAGAGTAACAAAAAATAATTTATAGAAAAG
TGATTAGTGTATTAGACCTGAGAGAGCCAGTGCAAGGCACTTAAGCTCACAGCGACATGA
ATCGTTCCTCTGGGAAAAAGCCGGTCATAGAACGAACCAGCAACGCCGCCAGCGAAGTAT
TTCGCCGGCGAGTCAACTTTCTGTTTCTCAGCCAACAACTTGAACAACATGTTGAAGTCA
TTAGAAGGCAAATCACAGAAGAAAGCCTCAAACTCCGGCGACCCACCACCACCGCACCTG
GCAGTGTATCGCCGTTGTAACGTCTCAACCACCGTATCAACGGTAGTAAACGTGTTGGAG
CCGGTGGCGCAGCCGAGATCGGTGATTTTGATGGGAGTTTGGTCGGAGAAGAGTTTGATG
GATTGGAGAGATGAGAAAGGGATTGGT

Last Updated on 06.12.2018 16:11