Genotyping and primer details


Genotyping and primer details for SALK_075550 - At5g60360
Line ID SALK_075550
Locus At5g60360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24280869 of line SALK_075550 Chr5:24280869
   
Insertion allele amplicon LBb3 - 27jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27jd: 5'-AGTTATTGAAAGCTCCAGCACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24281400
   
wt amplicon (over InsPos) 06km - 27jd
wt amplicon start Chr5:24280328
wt amplicon end Chr5:24281400
wt amplicon size 1073 bp
Locus specific primer 27jd: 5'-AGTTATTGAAAGCTCCAGCACAAT-3'
2nd locus specific primer 06km: 5'-AAAAGTATCAGAACGTGGAGGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11