Genotyping and primer details


Genotyping and primer details for SALK_075679c - At2g20850
Line ID SALK_075679c
Locus At2g20850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8975701 of line SALK_075679c Chr2:8975701
   
Insertion allele amplicon R204 - 05as
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 05as: 5'-AATGAACATGATGCATACCAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8974910
   
wt amplicon (over InsPos) 35lt - 05as
wt amplicon start Chr2:8974910
wt amplicon end Chr2:8976270
wt amplicon size 1361 bp
Locus specific primer 05as: 5'-AATGAACATGATGCATACCAAAAA-3'
2nd locus specific primer 35lt: 5'-CCAAGCTAGTTTGTGCTTGTTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11