Genotyping and primer details


Genotyping and primer details for SALK_076386c - At2g47090
Line ID SALK_076386c
Locus At2g47090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19342768 of line SALK_076386c Chr2:19342768
   
Insertion allele amplicon LBb3 - 13vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13vt: 5'-AAGCTTGAGAAGAACCAGAAGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19342466
   
wt amplicon (over InsPos) 19wq - 13vt
wt amplicon start Chr2:19342466
wt amplicon end Chr2:19343540
wt amplicon size 1075 bp
Locus specific primer 13vt: 5'-AAGCTTGAGAAGAACCAGAAGTTG-3'
2nd locus specific primer 19wq: 5'-AGTAGTGGTGACTCGGAAGTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11