Genotyping and primer details


Genotyping and primer details for SALK_076722 - At5g40370
Line ID SALK_076722
Locus At5g40370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16148498 of line SALK_076722 Chr5:16148498
   
Insertion allele amplicon R204 - CG97
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG97: 5'-TTTGTTTGTGAATCTTCGAATCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16148942
   
Insertion allele amplicon LBb3 - CG98
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG98: 5'-GAATTTGGCTACCATCACCTGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16148117
   
wt amplicon (over InsPos) CG98 - CG97
wt amplicon start Chr5:16148117
wt amplicon end Chr5:16148942
wt amplicon size 826 bp
Locus specific primer CG97: 5'-TTTGTTTGTGAATCTTCGAATCTG-3'
2nd locus specific primer CG98: 5'-GAATTTGGCTACCATCACCTGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11