Genotyping and primer details


Genotyping and primer details for SALK_077211c - At1g10760
Line ID SALK_077211c
Locus At1g10760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3586850 of line SALK_077211c Chr1:3586850
   
Insertion allele amplicon LBb3 - 54dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54dl: 5'-GGGACAGTAGGAGTAATAGGGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3586180
   
wt amplicon (over InsPos) 13ee - 54dl
wt amplicon start Chr1:3586180
wt amplicon end Chr1:3587252
wt amplicon size 1073 bp
Locus specific primer 54dl: 5'-GGGACAGTAGGAGTAATAGGGACC-3'
2nd locus specific primer 13ee: 5'-GGTTTCTCTTCCTTCTTGGTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11