Genotyping and primer details


Genotyping and primer details for SALK_078233 - At5g47050
Line ID SALK_078233
Locus At5g47050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19107464 of line SALK_078233 Chr5:19107464
   
Insertion allele amplicon LBb3 - 48vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48vi: 5'-GGTGGCTATGTACATCAAACCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19108018
   
wt amplicon (over InsPos) 63vw - 48vi
wt amplicon start Chr5:19106624
wt amplicon end Chr5:19108018
wt amplicon size 1395 bp
Locus specific primer 48vi: 5'-GGTGGCTATGTACATCAAACCTCT-3'
2nd locus specific primer 63vw: 5'-GCTCATCACCATCCTTCTACTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11