Sequences from confirmation amplicon


Confirmation sequences for line SALK_078233 and hit At5g47050 are shown.
Line ID SALK_078233
Locus At5g47050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19107464
Primers used for PCR 48vi - LBb3, show primer details
   
Sequence Name 51-K117945-SALK_078233C-48vi
GenBank Accession LN485542 [GenBank]
Sequencing primer gene specific primer, 48vi: 5'-GGTGGCTATGTACATCAAACCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117945-SALK_078233C-48vi
CAGTATACTACATATATAGTTTATATAAATGATAAATTTAATTTATATTTTTATTATTAT
TATTAAATTCCATTTGATTGGGCCTGATAAGCCCATTATAGAATGGCCCATCAAGAATAG
AAAACCTCCACGCTTGAATTCTTCGTGCTAAGGCAAAGCGGACAAATTCTCACGGAGCCG
TCGCATTCTGTACACATAACCAAATGACGACACGGCAAAGCCAACACCGTCGCCGATCGC
CGCCGGCAAATCCTACATCCCGGTCCAATCATTTCGACCCGGTCGGGATCCACATAAGCC
GATTCGGCGTCTTCAGCTTCATCCACACCGTCTACGCTTCCCGATTGTGGCTCCACCGTC
GTAATAACTCCGCCACCGTGAGCTACTACTACGGCTTGGTGTAATTGAGCTTGTAACGAC
GTCGCTTCGGCTTCTCTAGCAGCTGCTCTCATTTGCCAAGCACGTGCCTCTGTTTCGATC
TGAGTCGCACGTGCTTCTAGCTCGACAGGATATATTGTGGTGTAAACAAATTGACGCTTA
GACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAG
CGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 16-K117944-SALK_078233C-R210
GenBank Accession LN484728 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117944-SALK_078233C-R210
GTCATTTGTTACCCACAATATATCCTGTCGAGCTAGAAGCACGTGCGACTCAGATCGAAA
CAGAGGCACGTGCTTGGCAAATGAGAGCAGCTGCTAGAGAAGCCGAAGCGACGTCGTTAC
AAGCTCAATTACACCAAGCCGTAGTAGTAGCTCACGGTGGCGGAGTTATTACGACGGTGG
AGCCACAATCGGGAAGCGTAGACGGTGTGGATGAAGCTGAAGACGCCGAATCGGCTTATG
TGGATCCCGACCGGGTCGAAATGATTGGACCGGGATGTAGGATTTGCCGGCGGCGATCGG
CGACGGTGTTGGCTTTGCCGTGTCGTCATTTGGTTATGTGTACAGAATGCGACGGCTCCG
TGAGAATTTGTCCGCTTTGCCTTAGCACGAAGAATTCAAGCGTGGAGGTTTTCTATTCTT
GATGGGCCATTCTATAATGGGCTTATCAGGCCCAATCAAATGGAATTTAATAATAATAAT
AAAAATATAAATTAAATTTATCATTTATATAAACTATATATGTAGTATACTGTTGAGCTC
ATTATTGAATAGCTTGAGAGGTTTGATGTACATAGCCACC

Last Updated on 06.12.2018 16:11