Genotyping and primer details


Genotyping and primer details for SALK_078797c - At5g46590
Line ID SALK_078797c
Locus At5g46590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18906305 of line SALK_078797c Chr5:18906305
   
Insertion allele amplicon LBb3 - 31dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31dh: 5'-GAAAATCAAAGTCTTGTTGGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18906671
   
wt amplicon (over InsPos) 10ee - 31dh
wt amplicon start Chr5:18905942
wt amplicon end Chr5:18906671
wt amplicon size 730 bp
Locus specific primer 31dh: 5'-GAAAATCAAAGTCTTGTTGGAAGC-3'
2nd locus specific primer 10ee: 5'-ACATGGAATGGTACTTCTTCTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11