Genotyping and primer details


Genotyping and primer details for SALK_079096 - At3g60320
Line ID SALK_079096
Locus At3g60320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22294191 of line SALK_079096 Chr3:22294191
   
Insertion allele amplicon LBb3 - 27sr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27sr: 5'-CGTTATAGAGGCCTTTGTCTTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22293556
   
wt amplicon (over InsPos) 21tf - 27sr
wt amplicon start Chr3:22293556
wt amplicon end Chr3:22294568
wt amplicon size 1013 bp
Locus specific primer 27sr: 5'-CGTTATAGAGGCCTTTGTCTTGTC-3'
2nd locus specific primer 21tf: 5'-TCAGAGTATGATTTCTTCGACACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11