Sequences from confirmation amplicon


Confirmation sequences for line SALK_079096 and hit At3g60320 are shown.
Line ID SALK_079096
Locus At3g60320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22294191
Primers used for PCR 27sr - LBb3, show primer details
   
Sequence Name 20-K116528-SALK_079096C-27sr
GenBank Accession LN484860 [GenBank]
Sequencing primer gene specific primer, 27sr: 5'-CGTTATAGAGGCCTTTGTCTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116528-SALK_079096C-27sr
GCTTTGAGCTGTGATAACTTCTTCTCATGCTCAATCTTGAAACCTTCTCTAGCCTGGAAA
AAGAAATCATCAGCTACCAACAATCAATTGTTAAATTTCATAAAAGGAAAAACCTTTGAT
TTGGACAAACTGTAAAAGACCTTAATTTCTTCATAAAGCTTCTTCTCCCATGCCAAGAGA
CGGTCAAGAGTGGAACAAAGGCTCTTCGAACTGTTCGGTTGATCCAGCGCAGTCGTGTCT
ATCCTATACTTGACTGCCAATGGCGGCTTCGAGGTCCATGTCGAGCTCAAGTTGCTTAAT
AAACTACTCGAATGAATCACCGTTTCTAATAAACAAAATTCACAATGTCACAAACTCAAT
TTCATAAAAGCAGGATAATCCAAGTTATCAAAGAAAGAAAACAGAACGAACAATAGAACT
CACTCTTCAATTGGCTGAAACTACGATCGAGCTCAGCTCTGCCAAGCTCGAGCATCTGAG
AGACTTGCTCCCCTGAGGCAGCAGCCTTATCGAAATTTTCCTTAATGGCATCGATGATTT
CTTTCAAATCCCTATGCCTAACTACCATCTTCATGTCATAACACATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 12-K116527-SALK_079096C-R210
GenBank Accession LN484622 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116527-SALK_079096C-R210
TTATGACATGAAGATGGTAGTTAGGCATAGGGATTTGAAAGAAATCATCGATGCCATTAA
GGAAAATTTCGATAAGGCTGCTGCCTCAGGGGAGCAAGTCTCTCAGATGCTCGAGCTTGG
CAGAGCTGAGCTCGATCGTAGTTTCAGCCAATTGAAGAGTGAGTTCTATTGTTCGTTCTG
TTTTCTTTCTTTGATAACTTGGATTATCCTGCTTTTATGAAATTGAGTTTGTGACATTGT
GAATTTTGTTTATTAGAAACGGTGATTCATTCGAGTAGTTTATTAAGCAACTTGAGCTCG
ACATGGACCTCGAAGCCGCCATTGGCAGTCAAGTATAGGATAGACACGACTGCGCTGGAT
CAACCGAACAGTTCGAAGAGCCTTTGTTCCACTCTTGACCGTCTCTTGGCATGGGAGAAG
AAGCTTTATGAAGAAATTAAGGTCTTTTACAGTTTGTCCAAATCAAAGGTTTTTCCTTTT
ATGAAATTTAACAATTGATTGTTGGTAGCTGATGATTTCTTTTTCCAGGCTAGAGAAGGT
TTCAAGATTGAGCATGAGAAGAAGTTATCACAGCTTCAAAGCCAAGAATATAAAGGGGAG
GATGAAGCTAAGTTAGACAAGACAAAGGCTCCTTATAACG

Last Updated on 06.12.2018 16:11