Genotyping and primer details


Genotyping and primer details for SALK_079990c - At2g05090
Line ID SALK_079990c
Locus At2g05090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1818584 of line SALK_079990c Chr2:1818584
   
Insertion allele amplicon LBb3 - 27hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27hd: 5'-CATGGTGTTTGAAAATAATGCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:4549013
   
wt amplicon (over InsPos) 03ix - 27hd
wt amplicon start Chr2:1818198
wt amplicon end Chr2:4549013
wt amplicon size 744 bp
Locus specific primer 27hd: 5'-CATGGTGTTTGAAAATAATGCAAC-3'
2nd locus specific primer 03ix: 5'-GAGGATCGATTTCGTCAACTACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11