Genotyping and primer details


Genotyping and primer details for SALK_080154c - At5g43590
Line ID SALK_080154c
Locus At5g43590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17511767 of line SALK_080154c Chr5:17511767
   
Insertion allele amplicon LBb3 - 85cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 85cs: 5'-GAAACAAGAATCTCACACGTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17511080
   
wt amplicon (over InsPos) 17dz - 85cs
wt amplicon start Chr5:17511080
wt amplicon end Chr5:17511851
wt amplicon size 772 bp
Locus specific primer 85cs: 5'-GAAACAAGAATCTCACACGTCAAA-3'
2nd locus specific primer 17dz: 5'-CGAATCTCCCACTCATTTAGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11