Genotyping and primer details


Genotyping and primer details for SALK_080513c - At2g35710
Line ID SALK_080513c
Locus At2g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15012262 of line SALK_080513c Chr2:15012262
   
Insertion allele amplicon LBb3 - 24ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24ht: 5'-CCATGAGAAACCTAGTGGAAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15011569
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11