Genotyping and primer details


Genotyping and primer details for SALK_080688c - At4g38950
Line ID SALK_080688c
Locus At4g38950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18154756 of line SALK_080688c Chr4:18154756
   
Insertion allele amplicon LBb3 - 28hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28hd: 5'-CTAGTATCCGGACTTTCGTTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18155431
   
wt amplicon (over InsPos) 08il - 28hd
wt amplicon start Chr4:18154557
wt amplicon end Chr4:18155431
wt amplicon size 875 bp
Locus specific primer 28hd: 5'-CTAGTATCCGGACTTTCGTTGAAG-3'
2nd locus specific primer 08il: 5'-TTCTGCTACAAGAAACCCAAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11