Genotyping and primer details


Genotyping and primer details for SALK_080927c - At1g26650
Line ID SALK_080927c
Locus At1g26650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9210980 of line SALK_080927c Chr1:9210980
   
Insertion allele amplicon LBb3 - 52cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52cy: 5'-CCGACAAAATTGGAAATCTTACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9210112
   
wt amplicon (over InsPos) 34lt - 52cy
wt amplicon start Chr1:9210112
wt amplicon end Chr1:9211266
wt amplicon size 1155 bp
Locus specific primer 52cy: 5'-CCGACAAAATTGGAAATCTTACAT-3'
2nd locus specific primer 34lt: 5'-ACTCGGCAGCTAAAATAGAACACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11